Description
Boster’s human CSF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF1R has been precoated onto 96-well plates. Standards(NSO, I20-E512) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF1R amount of sample captured in plate.
Synonyms: C FMS|C FMS|CD 115|CD115|CD115 antigen|CFMS|Colony stimulating factor 1 receptor|CSF 1 R|CSF 1R|CSF-1 receptor|CSF-1-R|CSF1 R|CSF1R|CSF1R_HUMAN|CSFR|FIM 2|FIM2|FMS|FMS proto oncogene|FMS protooncogene|HDLS|M-CSF Receptor|M-CSF-R|Macrophage colony stimulating factor 1 receptor|Macrophage colony stimulating factor I receptor|Macrophage colony-stimulating factor 1 receptor|McDonough feline sarcoma viral (v fms) oncogene homolog|Oncogen FMS|Proto-oncogene c-Fms|V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
Species: Human CSF1R
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CSF1R
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, I20-E512
Gene name: Macrophage colony-stimulating factor 1 receptor / colony stimulating factor 1 receptor
Protein name: Macrophage colony-stimulating factor 1 receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|131|701|1183|140|669|1090
Standard deviation|5.76|55.37|67.43|6.58|60.21|70.85
CV(%)|4.4%|7.9%|5.7%|4.7%|9%|6.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 131 | 150 | 147 | 139 | 141 | 7.39 | 5.2% |
| Sample 2 | 701 | 763 | 743 | 706 | 728 | 25.8 | 3.5% |
| Sample 3 | 1183 | 1246 | 1399 | 1382 | 1302 | 90.97 | 6.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0807-CAP|96-well plate precoated with anti- human CSF1R antibody|1
EK0807-ST|Lyophilized recombinant human CSF1R standard|10ng/tube×2
EK0807-DA|Biotinylated anti- human CSF1R antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
