Description
Boster’s human IL-17RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-17RA has been precoated onto 96-well plates. Standards (NSO, L33- W320) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RA amount of sample captured in plate.
Synonyms: CANDF5|CD217|CD217 antigen|CDw217|CTLA8|HIL 17R|I17RA_HUMAN|IL 17 receptor A|IL 17 receptor|IL 17RA|IL-17 receptor A|IL-17RA|IL17|
IL17A receptor|IL17R|IL17RA|Interleukin 17 receptor A|Interleukin-17 receptor A|MGC10262
Species: Human IL-17RA
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IL-17RA
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L33-W320
Gene name: Interleukin-17 receptor A / interleukin 17 receptor A
Protein name: Interleukin-17 receptor A
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|84|276|1012|83|250|1067
Standard deviation|6.63|19.32|72.86|7.3|19.25|87.49
CV(%)|7.9%|7%|7.2%|8.8%|7.7%|8.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 78 | 90 | 82 | 83 | 4.33 | 5.2% |
| Sample 2 | 276 | 303 | 258 | 288 | 281 | 16.48 | 5.8% |
| Sample 3 | 1012 | 1007 | 960 | 871 | 962 | 56.58 | 5.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0784-CAP|96-well plate precoated with anti- human IL-17RA antibody|1
EK0784-ST|Lyophilized recombinant human IL-17RA standard|10ng/tube×2
EK0784-DA|Biotinylated anti- human IL-17RA antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
