Description
Boster’s human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(sf21, D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HBEGF amount of sample captured in plate.
Synonyms: Diphtheria toxin receptor|diphtheria toxin receptor (heparin-binding EGF-like growth factor)|diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)|DT R|DT-R|DTR|DTS|DTSF|HB-EGF|HBEGF|HBEGF_HUMAN|HEGFL|Heparin binding EGF like growth factor|Heparin binding epidermal growth factor|Heparin binding epidermal growth factor like growth factor|Heparin-binding EGF-like growth factor|Proheparin binding EGF like growth factor
Species: Human HBEGF
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human HBEGF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: sf21, D63-L148
Gene name: Proheparin-binding EGF-like growth factor / heparin-binding EGF-like growth factor
Protein name: Proheparin-binding EGF-like growth factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|16|77|282|15|70|280
Standard deviation|1|3.77|11.56|1.05|4.2|11.76
CV(%)|6.3%|4.9%|4.1%|7%|6%|4.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 14 | 15 | 15 | 15 | 0.7 | 4.6% |
| Sample 2 | 77 | 73 | 73 | 83 | 76 | 4.09 | 5.3% |
| Sample 3 | 282 | 268 | 286 | 279 | 278 | 6.68 | 2.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0770-CAP|96-well plate precoated with anti- human HBEGF antibody|1
EK0770-ST|Lyophilized recombinant human HBEGF standard|10ng/tube×2
EK0770-DA|Biotinylated anti- human HBEGF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
