Description
Boster’s human FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Y25-I251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF23 amount of sample captured in plate.
Synonyms: Fibroblast growth factor 23; FGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor; Fibroblast growth factor 23 N-terminal peptide; Fibroblast growth factor 23 C-terminal peptide; FGF23; HYPF; UNQ3027/PRO9828
Species: Human FGF23
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human FGF23
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,Y25-I251
Gene name: Fibroblast growth factor 23 / fibroblast growth factor 23
Protein name: Fibroblast growth factor 23
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|40|254|441|43|245|473
Standard deviation|3|10.16|30.42|3.74|9.8|35
CV(%)|7.5%|4%|6.9%|8.7%|4%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 33 | 38 | 36 | 36 | 2.58 | 7.1% |
| Sample 2 | 254 | 252 | 258 | 248 | 253 | 3.6 | 1.4% |
| Sample 3 | 441 | 455 | 471 | 515 | 470 | 27.79 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK0759-CAP|96-well plate precoated with anti- human FGF23 antibody|1
EK0759-ST|Lyophilized recombinant human FGF23 standard|10ng/tube×2
EK0759-DA|Biotinylated anti- human FGF23 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
