Description
Boster’s human CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD40 has been precoated onto 96-well plates. Standards(NSO, E21-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD40 amount of sample captured in plate.
Synonyms: AI326936|B cell associated molecule|B cell associated molecule CD40|B cell surface antigen CD40|Bp50|CD 40|CD40 antigen|CD40 antigen (TNF receptor superfamily member 5)|CD40 molecule|CD40 molecule TNF receptor superfamily member 5|CD40 protein|CD40 type II isoform|CD40L receptor|CDw40|GP39|HIGM1|IGM|MGC9013|Nerve growth factor receptor related B lymphocyte activation molecule|NFRSF5|OTTHUMP00000031699|OTTHUMP00000031700|p50|T BAM|TBAM|TNF receptor superfamily member 5|TNFRSF5|TRAP|Tumor necrosis factor receptor superfamily member 5|Tumor necrosis factor receptor superfamily member 5 precursor|Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
Species: Human CD40
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human CD40
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E21-R193
Gene name: Tumor necrosis factor receptor superfamily member 5 / CD40 molecule, TNF receptor superfamily member 5
Protein name: Tumor necrosis factor receptor superfamily member 5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|32|167|443|32|177|404
Standard deviation|1.66|9.18|34.99|1.92|10.79|38.78
CV(%)|5.2%|5.5%|7.9%|6%|6.1%|9.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 32 | 30 | 36 | 34 | 33 | 2.23 | 6.7% |
| Sample 2 | 167 | 185 | 189 | 177 | 179 | 8.41 | 4.6% |
| Sample 3 | 443 | 505 | 507 | 519 | 493 | 29.64 | 6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0702-CAP|96-well plate precoated with anti- human CD40 antibody|1
EK0702-ST|Lyophilized recombinant human CD40 standard|10ng/tube×2
EK0702-DA|Biotinylated anti- human CD40 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
