Description
Boster’s human CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL28 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: I23-Y127) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL28 amount of sample captured in plate.
Synonyms: CCK 1|CCK1|CCL 28|Chemokine (C-C motif) ligand 28|MEC|MGC71902|Mucosae associated epithelial chemokine|OTTHUMP0000022162|SCYA28|Small inducible cytokine A28|Small inducible cytokine A28 [Precursor]|Small inducible cytokine subfamily A (Cys-Cys), member 28
Species: human CCL28
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CCL28
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, I23-Y127
Gene name: C-C motif chemokine 28 / chemokine (C-C motif) ligand 28
Protein name: C-C motif chemokine 28
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|73|277|866|77|254|867
Standard deviation|5.03|21.32|59.75|5.77|20.32|64.15
CV(%)|6.9%|7.7%|6.9%|7.5%|8%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 73 | 82 | 79 | 69 | 75 | 5.06 | 6.7% |
| Sample 2 | 277 | 266 | 257 | 276 | 269 | 8.15 | 3% |
| Sample 3 | 866 | 893 | 781 | 873 | 853 | 42.87 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0690-CAP|96-well plate precoated with anti- human CCL28 antibody|1
EK0690-ST|Lyophilized recombinant human CCL28 standard|10ng/tube×2
EK0690-DA|Biotinylated anti- human CCL28 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
