Description
Boster’s rat MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-2 has been precoated onto 96-well plates. Standards(NSO, A30-C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-2 amount of sample captured in plate.
Synonyms: 72 kDa gelatinase|72 kDa type IV collagenase|CLG 4|CLG 4A|CLG4|CLG4A|Collagenase type IV A|EC 3.4.24.24|Gelatinase A|Gelatinase alpha|Gelatinase neutrophil|Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|Matrix metalloproteinase 2|Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)|Matrix metalloproteinase II|Matrix metalloproteinase-2|MMP 2|MMP II|MMP-2|MMP2|MMP2_HUMAN|MONA|Neutrophil gelatinase|PEX|TBE 1|TBE-1
Species: Rat MMP-2
Assay: 625pg/ml-40000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat total MMP-2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, A30-C662
Gene name: 72 kDa type IV collagenase / matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Protein name: 72 kDa type IV collagenase
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1093|4492|23073|1098|4440|21240
Standard deviation|68.85|296.47|1107.5|90.03|368.52|1295.64
CV(%)|6.3%|6.6%|4.8%|8.2%|8.3%|6.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1093 | 1090 | 975 | 1117 | 1068 | 55.12 | 5.1% |
| Sample 2 | 4492 | 3849 | 4402 | 4495 | 4309 | 268.48 | 6.2% |
| Sample 3 | 23073 | 23113 | 22919 | 26456 | 23890 | 1483.1 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0639-CAP|96-well plate precoated with anti- rat MMP-2 antibody|1
EK0639-ST|Lyophilized recombinant rat MMP-2 standard|40ng/tubex2
EK0639-DA|Biotinylated anti- rat MMP-2 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
