Description
Boster’s human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLGF has been precoated onto 96-well plates. Standards(E.coli, A21-R149) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLGF amount of sample captured in plate.
Synonyms: AI854365|_x000D_D12S1900|_x000D_MGC93298|_x000D__x000D__x000D_Pgf|_x000D_PGFL|_x000D_PIGF|_x000D_Placenta growth factor|_x000D_Placental growth factor|_x000D_Placental growth factor vascular endothelial growth factor related protein|_x000D_PlGF|_x000D_PlGF 2|_x000D_PLGF_HUMAN|_x000D_PlGF2|_x000D_SHGC 10760|_x000D_Vascular endothelial growth factor related protein
Species: Human PLGF
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human PLGF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, A21-R149
Gene name: Placenta growth factor / placental growth factor
Protein name: Placenta growth factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|40|221|454|37|215|459
Standard deviation|3.16|13.26|23.15|3.51|13.76|25.7
CV(%)|7.9%|6%|5.1%|9.5%|6.4%|5.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 38 | 37 | 42 | 39 | 1.92 | 4.9% |
| Sample 2 | 221 | 250 | 264 | 244 | 244 | 15.51 | 6.3% |
| Sample 3 | 454 | 469 | 445 | 509 | 469 | 24.49 | 5.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0490-CAP|96-well plate precoated with anti- human PLGF antibody|1
EK0490-ST|Lyophilized recombinant human PLGF standard|10ng/tubex2
EK0490-DA|Biotinylated anti- human PLGF antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
