Description
Boster’s mouse CCL19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL19 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: G26-S108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL19 amount of sample captured in plate.
Synonyms: Beta chemokine exodus 3|Beta-chemokine exodus-3|C C chemokine ligand 19|C-C motif chemokine 19|CC chemokine ligand 19|CCL19|CCL19_HUMAN|Chemokine (C C motif) ligand 19|Chemokine (CC motif) ligand 19|Chemokine CC Motif Ligand 19|CK beta 11|CK beta-11|CKb11|EBI1 ligand chemokine|ELC|Epstein-Barr virus-induced molecule 1 ligand chemokine|Exodus 3|Macrophage inflammatory protein 3 beta|MGC34433|MIP 3 beta|MIP-3-beta|MIP-3B|MIP3 beta|MIP3B|OTTHUMP00000000531|SCYA19|Small inducible cytokine A19|Small-inducible cytokine A19
Species: mouse CCL19
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CCL19
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, G26-S108
Gene name: C-C motif chemokine 19 / chemokine (C-C motif) ligand 19
Protein name: C-C motif chemokine 19
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|30|161|384|27|175|349
Standard deviation|1.2|8.53|29.56|1.45|9.27|33.5
CV(%)|4%|5.3%|7.7%|5.4%|5.3%|9.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 30 | 32 | 28 | 32 | 30 | 1.65 | 5.5% |
| Sample 2 | 161 | 166 | 173 | 167 | 166 | 4.26 | 2.5% |
| Sample 3 | 384 | 401 | 402 | 407 | 398 | 8.67 | 2.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0457-CAP|96-well plate precoated with anti- mouse CCL19 antibody|1
EK0457-ST|Lyophilized recombinant mouse CCL19 standard|10ng/tube×2
EK0457-DA|Biotinylated anti- mouse CCL19 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
