Description
Boster’s human MDC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MDC has been precoated onto 96-well plates. Standards(E.coli, G25-Q93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MDC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MDC amount of sample captured in plate.
Synonyms: A-152E5.1|ABCD 1|ABCD1|C-C motif chemokine 22|CC chemokine STCP-1|Ccl22|CCL22_HUMAN|Chemokine (C-C motif) ligand 22|DC/B-CK|Macrophage-derived chemokine|MDC(1-69)|MDC(7-69)|MGC34554|SCYA22|Small inducible cytokine A22|Small inducible cytokine subfamily A (Cys-Cys), member 22|Small-inducible cytokine A22|STCP 1|STCP1|Stimulated T cell chemotactic protein 1|Stimulated T-cell chemotactic protein 1
Species: Human MDC
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human MDC
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, G25-Q93
Gene name: C-C motif chemokine 22 / chemokine (C-C motif) ligand 22
Protein name: C-C motif chemokine 22
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|97|212|842|87|210|866
Standard deviation|7.37|9.11|55.57|7.91|12.6|64.08
CV(%)|7.6%|4.3%|6.6%|9.1%|6%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 97 | 96 | 88 | 94 | 93 | 3.49 | 3.7% |
| Sample 2 | 212 | 195 | 228 | 218 | 213 | 11.98 | 5.6% |
| Sample 3 | 842 | 819 | 834 | 802 | 824 | 15.27 | 1.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0446-CAP|96-well plate precoated with anti- human MDC antibody|1
EK0446-ST|Lyophilized recombinant human MDC standard|10ng/tubex2
EK0446-DA|Biotinylated anti- human MDC antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
