Description
Boster’s rat ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards(NSO, Q28-T493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ICAM-1 amount of sample captured in plate.
Synonyms: BB2|_x000D_CD 54|_x000D_CD54|_x000D__x000D__x000D_CD54 antigen|_x000D_Cell surface glycoprotein P3.58|_x000D_Human rhinovirus receptor|_x000D_ICAM 1|_x000D_Intercellular adhesion molecule 1|_x000D_Major group rhinovirus receptor|_x000D_MALA2|_x000D_MGC6195|_x000D_MyD10|_x000D_P3.58|_x000D_Surface antigen of activated B cells
Species: Rat ICAM-1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant rat ICAM-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q28-T493
Gene name: Intercellular adhesion molecule 1 / intercellular adhesion molecule 1
Protein name: Intercellular adhesion molecule 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|51|231|787|51|242|855
Standard deviation|3.67|14.55|42.49|4.23|16.21|51.3
CV(%)|7.2%|6.3%|5.4%|8.3%|6.7%|6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 51 | 47 | 49 | 56 | 50 | 3.34 | 6.6% |
| Sample 2 | 231 | 227 | 275 | 247 | 245 | 18.86 | 7.6% |
| Sample 3 | 787 | 761 | 862 | 797 | 801 | 37.18 | 4.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0372-CAP|96-well plate precoated with anti- rat ICAM-1 antibody|1
EK0372-ST|Lyophilized recombinant rat ICAM-1 standard|10ng/tube×2
EK0372-DA|Biotinylated anti- rat ICAM-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
