Description
Boster’s human Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fractalkine has been precoated onto 96-well plates. Standards(NSO, Q25-R339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fractalkine amount of sample captured in plate.
Synonyms: A 152E5.2|AB030188|ABCD 3|ABCD3|C X3 C motif chemokine 1|C3Xkine|Chemokine (C X3 C motif) ligand 1|Chemokine C X3 C motif ligand 1|Chemokine CX3C Motif Ligand 1|CX3C membrane anchored chemokine|CXC3|CXC3C|FKN|Fractalkine|Neurotactin|NTN|NTT|SCYD 1|SCYD1|Small inducible cytokine D1|Small inducible cytokine subfamily D (Cys X3 Cys), member 1|Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)|Small inducible cytokine subfamily D member 1
Species: Human Fractalkine
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human Fractalkine
Cross Reactivity: There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC< 0.2%. Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q25-R339
Gene name: Fractalkine / chemokine (C-X3-C motif) ligand 1
Protein name: Fractalkine
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|296|1773|5345|269|1730|5847
Standard deviation|16.87|90.42|315.35|17.75|103.8|374.2
CV(%)|5.7%|5.1%|5.9%|6.6%|6%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 296 | 303 | 298 | 261 | 289 | 16.65 | 5.7% |
| Sample 2 | 1773 | 1559 | 1657 | 1549 | 1634 | 90.41 | 5.5% |
| Sample 3 | 5345 | 5189 | 5317 | 5757 | 5402 | 213.22 | 3.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0356-CAP|96-well plate precoated with anti- human Fractalkine antibody|1
EK0356-ST|Lyophilized recombinant human Fractalkine standard|10ng/tube×2
EK0356-DA|Biotinylated anti- human Fractalkine antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
