Description
Boster’s human FLT3/FLK2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FLT3/FLK2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: N27-N541) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FLT3/FLK2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FLT3/FLK2 amount of sample captured in plate.
Synonyms: Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD135; FLT3; CD135; FLK2; STK1
Species: Human FLT3/FLK2
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human FLT3/FLK2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, N27-N541
Gene name: Receptor-type tyrosine-protein kinase FLT3 / fms related tyrosine kinase 3
Protein name: Receptor-type tyrosine-protein kinase FLT3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|73|300|1365|82|381|1527
Standard deviation|3.50|14.70|64.16|5.41|24.38|105.36
CV(%)|4.8%|4.9%|4.7%|6.6%|6.4%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 73 | 72 | 68 | 77 | 72 | 3.20 | 4.4% |
| Sample 2 | 300 | 276 | 286 | 285 | 286 | 8.58 | 3.0% |
| Sample 3 | 1365 | 1410 | 1476 | 1562 | 1453 | 74.16 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0352-CAP|96-well plate precoated with anti- human FLT3/FLK2 antibody|1
EK0352-ST|Lyophilized recombinant human FLT3/FLK2 standard|10ng/tube×2
EK0352-DA|Biotinylated anti- human FLT3/FLK2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
