Immunogen: Peptide corresponding to the hinge region of human IgG4 • Host: Rabbit • Species Reactivity: (+) Human • Cross Reactivity: (+) Human IgG4; (-) Human IgG1

IgG4 (human) Monoclonal Antibody – 100 µg

Brand:
Cayman
Storage:

Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.{28520} It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.{28520,55170,55174} IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.{28520} The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.{55171} IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.{55170} IgG exists as four isotypes in humans, IgG1, IgG2, IgG3, and IgG4, each of which has a distinct effector function. IgG4 is the least predominant IgG isotype in human serum and contains a serine at position 228 in its hinge region that facilitates Fab arm exchange, resulting in two antigen-binding sites and a functionally monovalent antibody.{53943} Increased serum levels of IgG4 positively correlate with desensitization and allergen tolerance in beekeepers, laboratory workers chronically exposed to rodent allergens, and individuals undergoing allergy immunotherapy for severe allergies to cats, dust mites, birch pollen, and wasps. Cayman’s IgG4 (human) Monoclonal Antibody can be used for ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes whole IgG4 at approximately 150 kDa under non-reducing conditions and the IgG4 heavy chain at approximately 50 kDa under reducing conditions from human samples.  

 

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SKU: 32123 - 100 µg Category:

Description

Immunogen: Peptide corresponding to the hinge region of human IgG4 • Host: Rabbit • Species Reactivity: (+) Human • Cross Reactivity: (+) Human IgG4; (-) Human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE; (-) Cynomolgus monkey, goat, mouse, rat, or rhesus monkey IgG • Applications: ELISA, FC, ICC, IHC, WB • MW = 150 and 50 kDa for whole IgG4 and the IgG4 heavy chain under non-reducing and reducing conditions, respectively

Synonyms: Immunoglobulin G4

Immunogen: Peptide corresponding to the hinge region of human IgG4

Formulation: 100 µg of protein A-affinity purified monoclonal antibody

Isotype: IgG

Applications: ELISA, ICC, IHC, WB

Origin:

Stability: 365 days

Application|ELISA||Application|Flow Cytometry||Application|Immunocytochemistry||Application|Immunohistochemistry||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Cardiovascular System|Blood|Serum Proteins||Research Area|Immunology & Inflammation|Adaptive Immunity||Research Area|Immunology & Inflammation|Allergy||Research Area|Infectious Disease

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