Methylation of key biological molecules and proteins plays important roles in numerous biological systems

Methyltransferase Colorimetric Assay Kit – 96 wells

Brand:
Cayman
Storage:
-80

Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing, and chromatin regulation.{16924} The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most commonly used enzymatic cofactor after ATP. SAM, also known as AdoMet, acts as a donor of a methyl group that is required for the modification of proteins and DNA.{16845} Aberrant levels of SAM have been linked to many abnormalities, including Alzheimer’s Disease, depression, Parkinson’s Disease, multiple sclerosis, liver failure, and cancer.{16924,16845} Cayman’s Methyltransferase Colorimetric Assay Kit is a continuous enzyme-coupled assay that can continuously monitor SAM-dependent methyltransferases.{15387} The removal of the methyl group from SAM generates S-adenosylhomocysteine (AdoHcy), which is rapidly converted to S-ribosylhomocysteine and adenine by AdoHcy nucleosidase. This rapid conversion prevents the buildup of AdoHcy and its feedback inhibition on the methylation reaction. Finally, the adenine is converted to hypoxanthine, by adenine deaminase, which in turn is converted to urate and hydrogen peroxide (H2O2). The rate of production of H2O2 is measured with the colorimetric reagent, 3,5-dichloro-2-hydroxybenzenesulfonic acid, by an increase in absorbance at 500-520 nm. The assay is supplied with AdoHcy as a positive control. The assay can be used with any purified SAM-dependent methyltransferase.  

 

Available on backorder

Add to quotes
SKU: 700140 - 96 wells Category:

Description

Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing, and chromatin regulation. The SAM dependent MTs use SAM as the enzymatic cofactor. SAM, also known as AdoMet, acts as a donor of a methyl group that is required for the modification of proteins and DNA. Cayman’s MT Colorimetric Assay is a continuous enzyme-coupled assay that can continuously monitor SAM-dependent MT activities. The removal of the methyl group from SAM generates AdoHcy, which is rapidly converted to urate and H2O2 by an enzyme mixture provided in the kit. The rate of production of H2O2 is measured with the colorimetric reagent, 3,5-dichloro-2-hydroxybenzenesulfonic acid, by an increase in absorbance at 500-520 nm. The assay is supplied with AdoHcy as a positive control. The assay can be used with any purified SAM-dependent MT.

Formulation:

Formal name:

Synonyms:  MT

Host:

Imunogen:

Applications: 

Clone:

Purity:

Origin:

Product Type|Assay Kits|Colorimetric Assays||Product Type|Assay Kits|Enzyme Activity||Research Area|Epigenetics, Transcription, & Translation|Writers|DNA/RNA Methylation

Related products