The Glutathione Fluorescent Assay Kit is designed to measure the amount of total glutathione (GSH+GSSG) in cells and tissue homogenates and the total amount (including GSSG and protein mixed disulfides with glutathione) in plasma. The assay is simple and sensitive based on eosinGSH that emits a strong fluorescent signal detectable at 545 nm after excitation at 520 nm.[IMCO Catalog FkGSH-01]

Glutathione Fluorescent Detection Assay Kit – 96 wells

Brand:
Cayman
Storage:
-20

Glutathione (GSH) is a tripeptide (γ-Glu-Cys-Gly) present a very high concentrations throughout living organisms (from 1 to 10 nM) and exists in three major forms: reduced sulphydryl (GSH), glutathione disulfide (GSSG), or bound to Cys residues in proteins (PSSG). From its first described role in heavy metals and xenobiotics detoxification, it is now known that GSH plays a key role in cell redox homeostasis, gene expression, cell proliferation, and apoptosis mainly by binding to Cys residues in protein, known as protein glutathionylation (PSSG). GSH is used by glutaredoxin (Grx) to reduce disulfides. This reaction is usually coupled to the NADPH driven reduction of GSSG by glutathione reductase (GR). The ratio GSH/GSSG has been used as an indicator of the redox level in cells but this parameter can also be estimated by the quantification of PSSG. In fact, PSSG has the advantage of being more stable than GSSG. Within mammalian cells, glutathione exists mainly (>98%) in the thiol-reduced form (GSH). In fact the oxidized form, GSSG, is toxic in the cytosol and it is either reduced by glutathione reductase or exported from cells when its cytosolic concentration increases. Vice versa in plasma glutathione is present mainly as the oxidized form both as GSSG and mixed disulfides (Cysteinyl-glutathione and PSSG). This kit is designed to measure the amount of total glutathione (GSH+GSSG) in cells and tissue homogenates and the total amount (including GSSG and protein mixed disulfides with glutathione) in plasma.{35436} The assay is simple and sensitive based on eosinGSH that emits a strong fluorescent signal detectable at 545 nm after excitation at 520 nm.[IMCO Catalog FkGSH-01]  

 

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SKU: 26404 - 96 wells Category:

Description

The Glutathione Fluorescent Assay Kit is designed to measure the amount of total glutathione (GSH+GSSG) in cells and tissue homogenates and the total amount (including GSSG and protein mixed disulfides with glutathione) in plasma. The assay is simple and sensitive based on eosinGSH that emits a strong fluorescent signal detectable at 545 nm after excitation at 520 nm.[IMCO Catalog FkGSH-01]

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Synonyms:  GSH

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Application|Fluorescence||Product Type|Assay Kits|Fluorometric Assays||Research Area|Oxidative Stress & Reactive Species|Antioxidant Activity||Research Area|Oxidative Stress & Reactive Species|Reactive Sulfur|Glutathione

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