SREBP-2 is a transcription factor that performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase

SREBP-2 Transcription Factor Assay Kit – 96 wells

Brand:
Cayman
Storage:
-80

Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory element-binding proteins (SREBP’s). SREBP’s directly activate the expression of over 30 genes involved in the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids.{14287} Three different SREBP isoforms designated SREBP-1a, SREBP-1c, and SREBP-2 are encoded by two different genes and belong to the basic helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors.{14286} SREBP-2 performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor. Cayman’s SREBP-2 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96 well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.  

 

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SKU: 10007819 - 96 wells Category:

Description

SREBP-2 is a transcription factor that performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor.

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Synonyms:  SREBF2|Sterol Regulatory Element-binding Protein-2|Sterol Regulatory Element-binding Transcription Factor 2

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Origin: Animal/Rabbit|Animal/Bovine|Animal/Goat

Product Type|Assay Kits|Colorimetric Assays||Product Type|Assay Kits|ELISA||Research Area|Cardiovascular System|Lipids & Lipoproteins|Cholesterol Metabolism||Research Area|Endocrinology & Metabolism|Metabolic Diseases|Dyslipidemias

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