A racemic version of a CYP450 metabolite of arachidonic acid; provided as a mixture of free acid and lactone; inhibits Cav3 channels (IC50 = 0.54 µM for Cav3.2); decreases nifedipine-resistant phenylephrine-induced vasoconstriction in isolated mouse mesenteric arteries via Cav3.2 blockade at 3 µM; a substrate of COX-1 and COX-2 in oxygen consumption and product formation assays at 50 µM

(±)5(6)-EET – 50 µg

Brand:
Cayman
CAS:
87173-80-6
Storage:
-80
UN-No:
De Minimis - 1231 / 3

5(6)-EET is a fully racemic version of the enantiomeric forms biosynthesized from arachidonic acid (Item No. 90010) by cytochrome P450 enzymes.{463,729} In solution, 5(6)-EET degrades into 5,6-DiHET and 5(6)-δ-lactone, which can be converted to 5(6)-DiHET and quantified by GC-MS.{39654} In neuroendocrine cells, such as the anterior pituitary and pancreatic islets, 5(6)-EET has been implicated in the mobilization of calcium and hormone secretion.{462,460} 5(6)-EET is an inhibitor of T-type voltage-gated calcium channels (Cav3) that inhibits isoforms Cav3.1, Cav3.2 (IC50 = 0.54 µM), and Cav3.3 and decreases nifedipine-resistant phenylephrine-induced vasoconstriction in isolated mouse mesenteric arteries via Cav3.2 blockade when used at a concentration of 3 µM.{42110} In addition, it is a substrate of COX-1 and COX-2, as measured by oxygen consumption and product formation assays when used at a concentration of 50 µM.{34481} (±)5(6)-EET is provided as a mixture of the free acid and lactone.  

 

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SKU: 50211 - 50 µg Category:

Description

A racemic version of a CYP450 metabolite of arachidonic acid; provided as a mixture of free acid and lactone; inhibits Cav3 channels (IC50 = 0.54 µM for Cav3.2); decreases nifedipine-resistant phenylephrine-induced vasoconstriction in isolated mouse mesenteric arteries via Cav3.2 blockade at 3 µM; a substrate of COX-1 and COX-2 in oxygen consumption and product formation assays at 50 µM

Formal name: (±)5,6-epoxy-8Z,11Z,14Z-eicosatrienoic acid

Synonyms:  (±)5,6-EpETrE

Molecular weight: 320.5

CAS: 87173-80-6

Purity: ≥95% (mixture of free acid and lactone)

Formulation: A solution in methyl acetate

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