Reacts with thiols resulting in an increase in fluorescence intensity of up to 120-fold; the response is selective for thiols

ThioFluor 623 – 5 mg

Brand:
Cayman
CAS:
1004324-99-5
Storage:
-20
UN-No:
Non-Hazardous - /

The rapid, selective, and sensitive sensing of thiols is important in diverse areas of research. This thiofluor 623 responds upon exposure to thiols with an increase in fluorescence intensity of up to 120-fold.{16211} The response is selective for thiols and occurs in aqueous media.{16211} In the absence of thiols, the probe is essentially non-fluorescent; thiols cause cleavage of the probe, generating a fluorophore with an absorption maximum of 563 nm and emission at 623 nm.{16211} The fluorescence quantum yield of the cleaved product, generated in response to thiols, increases in more viscous media, suggesting ideal performance in biological systems and applicability to single-molecule or 2-photon sensing schemes. The thiofluor 623 is cell-permeable and reacts selectively with intracellular thiols.{16211} The pseudo-first order rate constant (kobs) depends on substrate (e.g., 2.1 x 10−3 s−1 for cysteine, 2.0 x 10−5 s−1 for human serum albumin).{16211}  

 

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Description

Reacts with thiols resulting in an increase in fluorescence intensity of up to 120-fold; the response is selective for thiols, occurs in aqueous media, and results in a fluorophore with emission at 623 nm; cell-permeable

Formal name: N-[4-[(1E)-2-[4-cyano-5-(dicyanomethylene)-2,5-dihydro-2,2-dimethyl-3-furanyl]ethenyl]phenyl]-N-[2-[2-(2-methoxyethoxy)ethoxy]ethyl]2,4-dinitro-benzenesulfonamide

Synonyms:  Fluorescent Thiol Probe

Molecular weight: 678.7

CAS: 1004324-99-5

Purity: ≥98%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Labeling & Detection|Reactive Probes||Research Area|Oxidative Stress & Reactive Species|Reactive Sulfur

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