A cell-permeant fluorogenic dye used to assess mitochondrial function and cell viability; excitation/emission spectra 550/575 nm

Tetramethylrhodamine ethyl ester (perchlorate) – 10 mg

Brand:
Cayman
CAS:
115532-52-0
Storage:
-20
UN-No:
Non-Hazardous - /

Tetramethylrhodamine ethyl ester (TMRE) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.{34471,34474} It displays excitation/emission spectra of 550/575 nm, respectively. Due to the polarization of the mitochondrial membrane, TMRE is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.  

 

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Description

A cell-permeant fluorogenic dye used to assess mitochondrial function and cell viability; excitation/emission spectra 550/575 nm

Formal name: 3,6-bis(dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-xanthylium, perchlorate

Synonyms:  TMR ethyl ester|TMRE

Molecular weight: 515

CAS: 115532-52-0

Purity: ≥98%

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Application|Immunocytochemistry||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Mitochondrial Biology

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