An FH2 domain inhibitor; inhibits assembly of profilin-actin mediated by mouse formin mDia1(FH1FH2) (IC50 = ~15 μM); inhibits actin assembly mediated by FH1FH2 constructs of mDia2

SMIFH2 – 10 mg

Brand:
Cayman
CAS:
340316-62-3
Storage:
-20
UN-No:
De Minimis - 2811 / 6.1

SMIFH2 is an inhibitor of formin homology 2 (FH2) domains.{54322} It inhibits assembly of profilin-actin mediated by mouse formin mDia1(FH1FH2) with an IC50 value of approximately 15 μM. It also inhibits actin assembly mediated by FH1FH2 constructs of mDia2, C. elegans CYK-1, S. pombe Cdc12 or Fus1, or S. cerevisiae Bni1. SMIFH2 (25 μM) disassembles formin-dependent actin cables and contractile rings, but not Arp2/3-dependent actin patches, in fission yeast. It is cytotoxic to NIH3T3 fibroblasts (IC50 = 28 μM). SMIFH2 (10 μM) increases and decreases the percentage of NIH3T3 cells with thin and thick F-actin bundles, respectively.  

 

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SKU: 31517 - 10 mg Category:

Description

An FH2 domain inhibitor; inhibits assembly of profilin-actin mediated by mouse formin mDia1(FH1FH2) (IC50 = ~15 μM); inhibits actin assembly mediated by FH1FH2 constructs of mDia2, C. elegans CYK-1, S. pombe Cdc12 or Fus1, or S. cerevisiae Bni1; disassembles formin-dependent actin cables and contractile rings in fission yeast at 25 μM; cytotoxic to NIH3T3 fibroblasts (IC50 = 28 μM); increases and decreases the percentage of NIH3T3 cells with thin and thick F-actin bundles, respectively, at 10 μM

Formal name: 1-(3-bromophenyl)-5-(2-furanylmethylene)dihydro-2-thioxo-4,6(1H,5H)-pyrimidinedione

Synonyms: 

Molecular weight: 377.2

CAS: 340316-62-3

Purity: ≥98%

Formulation: A solid

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