An inhibitor of miR-544 biogenesis; increases MTOR mRNA expression and decreases the mRNA expression of the genes encoding miR-544

SID 3712249 – 25 mg

Brand:
Cayman
CAS:
522606-67-3
Storage:
-20
UN-No:
Non-Hazardous - /

SID 3712249 inhibits the biogenesis of microRNA-544 (miR-544), a silencer of mammalian target of rapamycin (mTOR).{54014} Under hypoxic conditions, SID 3712249 (20 nM) increases MTOR mRNA expression and decreases the mRNA expression of the genes encoding miR-544, hypoxia-inducible factor-1α (HIF-1α), and ataxia-telangiectasia mutated kinase (ATM) in MDA-MB-231, MCF-7, and MCF-10A cells. It induces apoptosis in MDA-MB-231 cells in a hypoxia- and mTOR-dependent manner when used at a concentration of 20 nM. SID 3712249 (20 nM) enhances the sensitivity of MCF-7 and MDA-MB-231 cells cultured under hypoxic conditions to decreases in cell viability induced by 5-fluorouracil (5-FU; Item No. 14416). It reduces tumor growth in an MDA-MB-231 mouse xenograft model.  

 

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Description

An inhibitor of miR-544 biogenesis; increases MTOR mRNA expression and decreases the mRNA expression of the genes encoding miR-544, HIF-1α, and ATM in MDA-MB-231, MCF-7, and MCF-10A cells under hypoxic conditions at 20 nM; induces apoptosis in MDA-MB-231 cells in a hypoxia- and mTOR-dependent manner at 20 nM; enhances the senstivity of MCF-7 and MDA-MB-231 cells cultured under hypoxic conditions to 5-FU-induced decreases in cell viability at 20 nM; reduces tumor growth in an MDA-MB-231 mouse xenograft model

Formal name: 1,8-diamino-3,6-di-1-pyrrolidinyl-2,7-naphthyridine-4-carbonitrile

Synonyms: 

Molecular weight: 323.4

CAS: 522606-67-3

Purity: ≥95%

Formulation: A crystalline solid

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