Description
This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) nanobody is a recombinant monoclonal antibody generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). Also, the DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA).
The SARS-CoV-2 S1-RBD Nanobody is also validated in Neutralizing and LSPR. In neutralizing assay, the binding signal of SARS-CoV-2 S1 RBD and ACE2 was inhibited by SARS-CoV-2 Spike RBD Nanobody. The IC50 is typically 0.1074 ug/ml. In the LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM).
Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.
SARS-CoV-2 Spike RBD Nanobody Product Details
| Uniprot No. | P0DTC2 | ||||||||
| Target Names | S (Spike glycoprotein) | ||||||||
| Species Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ||||||||
| Immunogen | Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) | ||||||||
| Immunogen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ||||||||
| Conjugate | Non-conjugated | ||||||||
| Clonality | Monoclonal | ||||||||
| Isotype | VHH fusion with human IgG1 Fc | ||||||||
| Clone No. | A1 | ||||||||
| Purification Method | Affinity-chromatography | ||||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
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| Form | Liquid | ||||||||
| Tested Applications | ELISA, GICA, Neutralising | ||||||||
| Recommended Dilution |
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| Protocols | ELISA Protocol CSB-RA33245A2GMY LSPR protocol |
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| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
