A more stable form of (–)-blebbistatin

(S)-nitro-Blebbistatin – 5 mg

Brand:
Cayman
CAS:
856925-75-2
Storage:
-20
UN-No:
Non-Hazardous - /

(S)-nitro-Blebbistatin is a more stable form of (–)-blebbistatin (Item No. 13013), which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.{16452,17037} (–)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50 = 80 µM).{17036} Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.{17038,24037} The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure.{16725} (S)-nitro-Blebbistatin has the same stereochemistry as the active (–)-blebbistatin enantiomer.  

 

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Description

A more stable form of (–)-blebbistatin, the active form of blebbistatin; has the same stereochemistry as the active (–)-blebbistatin enantiomer,

Formal name: (3aS)-1,2,3,3a-tetrahydro-3a-hydroxy-7-nitro-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one

Synonyms:  S-(−)-7-Desmethyl-8-nitro Blebbistatin

Molecular weight: 323.3

CAS: 856925-75-2

Purity: ≥98%

Formulation: A crystalline solid

Product Type|Biochemicals|Small Molecule Inhibitors|ATPases||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Cytoskeleton & Motor Proteins

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