Description
Boster’s rat VEGFR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR3 has been precoated onto 96-well plates. Standards(NSO, Y25-D770) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat VEGFR3 amount of sample captured in plate.
Synonyms: FLT 4|FLT4|FLT41|Fms like tyrosine kinase 4|Fms related tyrosine kinase 4|LMPH1A|PCL|Tyrosine protein kinase receptor FLT4|Vascular endothelial growth factor receptor 3|VEGFR 3|VEGFR3
Species: rat VEGFR3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat VEGFR3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, Y25‑D770
Gene name: Vascular endothelial growth factor receptor 3 / fms-related tyrosine kinase 4
Protein name: Vascular endothelial growth factor receptor 3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|256|1651|4864|259|1599|5230
Standard deviation|11.77|70.99|321.02|14.5|92.74|371.33
CV(%)|4.6%|4.3%|6.6%|5.6%|5.8%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 256 | 221 | 252 | 247 | 244 | 13.65 | 5.5% |
| Sample 2 | 1651 | 1702 | 1683 | 1684 | 1680 | 18.37 | 1% |
| Sample 3 | 4864 | 4765 | 4911 | 4762 | 4825 | 64.19 | 1.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1347-CAP|96-well plate precoated with anti- rat VEGFR3 antibody|1
EK1347-ST|Lyophilized recombinant rat VEGFR3 standard|10ng/tube×2
EK1347-DA|Biotinylated anti- rat VEGFR3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
