Description
Boster’s rat SCGB1A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: S20-V96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SCGB1A1 amount of sample captured in plate.
Synonyms: Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg
Species: Rat SCGB1A1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat SCGB1A1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,S20-V96
Gene name: Uteroglobin / secretoglobin family 1A member 1
Protein name: Uteroglobin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|220|1610|4490|241|1635|4849
Standard deviation|8.8|123.97|296.34|10.36|140.61|378.22
CV(%)|4%|7.7%|6.6%|4.3%|8.6%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 220 | 258 | 259 | 253 | 247 | 16.03 | 6.4% |
| Sample 2 | 1610 | 1483 | 1380 | 1552 | 1506 | 85.63 | 5.6% |
| Sample 3 | 4490 | 4875 | 4487 | 4849 | 4675 | 186.97 | 3.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1636-CAP|96-well plate precoated with anti- rat SCGB1A1 antibody|1
EK1636-ST|Lyophilized recombinant rat SCGB1A1 standard|10ng/tube×2
EK1636-DA|Biotinylated anti- rat SCGB1A1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
