Description
Boster’s rat Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rantes has been precoated onto 96-well plates. Standards(E.coli, S24-S91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Rantes amount of sample captured in plate.
Synonyms: Beta chemokine RANTES|Beta chemokine RANTES precursor|C C motif chemokine 5|CCL 5|CCL5|CCL5_HUMAN|Chemokine (C C motif) ligand 5|Chemokine CC Motif Ligand 5|D17S136E|EoCP|Eosinophil chemotactic cytokine|MGC17164|RANTES(4-68)|Regulated upon activation normally T expressed and presumably secreted|SCYA 5|SCYA5|SIS delta|SIS-delta|SISd|Small inducible cytokine A5|Small inducible cytokine A5 (RANTES)|Small inducible cytokine subfamily A (Cys Cys) member 5|Small-inducible cytokine A5|T cell specific protein p288|T cell specific protein RANTES|T cell specific RANTES protein|T cell-specific protein P228|T-cell-specific protein RANTES|TCP 228|TCP228
Species: Rat Rantes
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant rat Rantes
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, S24-S91
Gene name: C-C motif chemokine 5 / chemokine (C-C motif) ligand 5
Protein name: C-C motif chemokine 5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|34|208|374|37|194|349
Standard deviation|2.21|15.8|19.82|2.81|14.93|25.12
CV(%)|6.5%|7.6%|5.3%|7.6%|7.7%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 30 | 30 | 33 | 31 | 1.78 | 5.7% |
| Sample 2 | 208 | 182 | 192 | 203 | 196 | 10.05 | 5.1% |
| Sample 3 | 374 | 383 | 389 | 374 | 380 | 6.36 | 1.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0496-CAP|96-well plate precoated with anti- rat Rantes antibody|1
EK0496-ST|Lyophilized recombinant rat Rantes standard|10ng/tubex2
EK0496-DA|Biotinylated anti- rat Rantes antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
