Description
Boster’s rat RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RANK has been precoated onto 96-well plates. Standards(NSO, Q30-P213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RANK amount of sample captured in plate.
Synonyms: CD265|FEO|LOH18CR1|Loss of heterozygosity 18 chromosomal region 1|ODFR|OFE|OPTB7|Osteoclast differentiation factor receptor|OSTS|PDB2|Receptor activator of NF-KB|Receptor activator of nuclear factor kappa B|TNFRSF11A|TNR11_HUMAN|TRANCER|Tumor necrosis factor receptor superfamily member 11A|Tumor necrosis factor receptor superfamily member 11a activator of NFKB|Tumor necrosis factor receptor superfamily member 11a NFKB activator
Species: rat RANK
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat RANK
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, Q30-P213
Gene name: Tumor necrosis factor receptor superfamily member 11A / tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Protein name: Tumor necrosis factor receptor superfamily member 11A
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|209|514|1401|226|542|1518
Standard deviation|13.58|34.95|88.26|18.3|42.81|97.15
CV(%)|6.5%|6.8%|6.3%|8.1%|7.9%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 209 | 194 | 185 | 217 | 201 | 12.49 | 6.2% |
| Sample 2 | 514 | 489 | 548 | 539 | 522 | 23 | 4.4% |
| Sample 3 | 1401 | 1406 | 1464 | 1575 | 1461 | 70.05 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1281-CAP|96-well plate precoated with anti- rat RANK antibody|1
EK1281-ST|Lyophilized recombinant rat RANK standard|10ng/tube×2
EK1281-DA|Biotinylated anti- rat RANK antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
