Description
Boster’s rat MIP-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-1 alpha has been precoated onto 96-well plates. Standards(E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MIP-1 alpha amount of sample captured in plate.
Synonyms: C-C motif chemokine 3;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;Small-inducible cytokine A3;Ccl3;Mip1a, Scya3;
Species: rat MIP-1 alpha
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant rat MIP-1 alpha
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin, EDTA or Citrate
Immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-A92
Gene name: C-C motif chemokine 3 / C-C motif chemokine 3
Protein name: C-C motif chemokine 3
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 18 | 54 | 267 | 18 | 56 | 248 |
| Standard deviation | 1.08 | 3.61 | 15.75 | 1.11 | 4.48 | 14.88 |
| CV(%) | 6% | 6.7% | 5.9% | 6.2% | 8% | 6% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 18 | 18 | 18 | 16 | 17 | 0.86 | 5% |
| Sample 2 | 54 | 52 | 47 | 47 | 50 | 3.08 | 6.1% |
| Sample 3 | 267 | 269 | 269 | 304 | 277 | 15.46 | 5.5% |
*number of samples for each test n=16.
10ng/tube
