Description
Boster’s rat IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(P70) has been precoated onto 96-well plates. Standards (Expression system for standard: sf21, Immunogen sequence: (M23-S335)+(R23-S215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-12(P70) amount of sample captured in plate.
Synonyms: Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Il12a
Species: Rat IL-12(P70)
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1.5pg/ml Specifity: Natural and recombinant rat IL-12(P70)
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: sf21,(M23 – S335)+(R23 – S215)
Gene name: Interleukin-12 subunit alpha / interleukin 12A
Protein name: Interleukin-12 subunit alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|35|222|502|34|224|454
Standard deviation|1.92|15.76|27.61|2.07|19.93|29.05
CV(%)|5.5%|7.1%|5.5%|6.1%|8.9%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 31 | 36 | 34 | 34 | 1.87 | 5.5% |
| Sample 2 | 222 | 226 | 219 | 229 | 224 | 3.8 | 1.6% |
| Sample 3 | 502 | 447 | 478 | 479 | 476 | 19.55 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1652-CAP|96-well plate precoated with anti- rat IL-12(p70) antibody|1
EK1652-ST|Lyophilized recombinant rat IL-12(p70) standard|10ng/tube×2
EK1652-DA|Biotinylated anti-rat IL-12(p70) antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
