Description
Boster’s rat IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V117-S268) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1 beta amount of sample captured in plate.
Synonyms: Catabolin|_x000D_Hematopoietin 1|_x000D__x000D__x000D_IFN beta inducing factor|_x000D_IL 1|_x000D_IL 1 beta|_x000D_IL 1B|_x000D_IL-1 beta|_x000D_IL1|_x000D_IL1 BETA|_x000D_IL1B|_x000D_IL1B_HUMAN|_x000D_IL1F2|_x000D_Interleukin 1 beta|_x000D_Interleukin 1 beta precursor|_x000D_Interleukin-1 beta|_x000D_LAF|_x000D_OAF|_x000D_Osteoclast activating factor|_x000D_Preinterleukin beta|_x000D_Pro interleukin 1 beta
Species: Rat IL-1 beta
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant rat IL-1 beta
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,V117-S268
Gene name: Interleukin-1 beta / interleukin 1, beta
Protein name: Interleukin-1 beta
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|58|233|962|62|241|881
Standard deviation|3.65|11.41|69.26|4.21|12.29|68.71
CV(%)|6.3%|4.9%|7.2%|6.8%|5.1%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 59 | 57 | 56 | 57 | 1.11 | 1.9% |
| Sample 2 | 233 | 268 | 237 | 251 | 247 | 13.71 | 5.5% |
| Sample 3 | 962 | 826 | 929 | 964 | 920 | 56.16 | 6.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0393-CAP|96-well plate precoated with anti- rat IL-1 beta antibody|1
EK0393-ST|Lyophilized recombinant rat IL-1 beta standard|10ng/tubex2
EK0393-DA|Biotinylated anti- rat IL-1 beta antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
