Description
Boster’s rat FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(NSO, Q127-L278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat FASL amount of sample captured in plate.
Synonyms: Apoptosis (APO 1) antigen ligand 1|_x000D_Apoptosis antigen ligand|_x000D_APT1LG1|_x000D__x000D__x000D_APTL|_x000D_CD178|_x000D_CD95 L|_x000D_CD95 ligand|_x000D_CD95L|_x000D_CD95L protein|_x000D_Fas antigen ligand|_x000D_FASL|_x000D_FASLG|_x000D_TNF superfamily member 6|_x000D_TNFSF6|_x000D_Tumor necrosis factor (ligand) superfamily member 6|_x000D_Tumor necrosis factor ligand superfamily member 6
Species: rat FASL
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat FAS-L
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, Q127-L278
Gene name: Tumor necrosis factor ligand superfamily member 6 / Fas ligand (TNF superfamily, member 6)
Protein name: Tumor necrosis factor ligand superfamily member 6
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|78|266|931|83|263|1019
Standard deviation|3.97|11.17|40.03|4.31|12.88|61.14
CV(%)|5.1%|4.2%|4.3%|5.2%|4.9%|6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 78 | 71 | 76 | 77 | 75 | 2.69 | 3.5% |
| Sample 2 | 266 | 267 | 298 | 284 | 278 | 13.21 | 4.7% |
| Sample 3 | 931 | 902 | 822 | 837 | 873 | 45 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1110-CAP|96-well plate precoated with anti- rat FASL antibody|1
EK1110-ST|Lyophilized recombinant rat FASL standard|10ng/tube×2
EK1110-DA|Biotinylated anti- rat FASL antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
