Description
Boster’s rat Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Eotaxin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: H24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Eotaxin amount of sample captured in plate.
Synonyms: C-C motif chemokine 11|CCL 11|CCL11|CCL11_HUMAN|Chemokine (C-C motif) ligand 11|Chemokine C C motif ligand 11|Chemokine CC Motif Ligand 11|Chemokine ligand 11|Eosinophil Chemotactic P|Eosinophil chemotactic protein|Eotaxin|Eotaxin 1|SCYA 11|SCYA11
Species: rat Eotaxin
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat Eotaxin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA or citrate
Immunogen: E.coli, H24-P97
Gene name: Eotaxin / chemokine (C-C motif) ligand 11
Protein name: Eotaxin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|53|222|1050|58|464|1127
Standard|deviation|3.71|12.88|64.05|3.07|19.49|66.50
CV(%)|7%|5.8%|6.1%|5.3%|4.2%|5.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 53 | 51 | 51 | 53 | 52 | 1.00 | 1.9% |
| Sample 2 | 222 | 212 | 240 | 236 | 227 | 11.16 | 4.9% |
| Sample 3 | 1050 | 1017 | 957 | 1091 | 1028 | 49.02 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1435-CAP|96-well plate precoated with anti- rat Eotaxin antibody|1
EK1435-ST|Lyophilized recombinant rat Eotaxin standard|10ng/tube×2
EK1435-DA|Biotinylated anti- rat Eotaxin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
