Description
Boster’s rat CXCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL4 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: V30-S105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL4 amount of sample captured in plate.
Synonyms: Platelet factor 4; PF-4; C-X-C motif chemokine 4; Pf4; Cxcl4; Scyb4
Species: Rat CXCL4/PF4
Assay: 156pg/ml-10000pg/ml
Sensitivy: <15pg/ml Specifity: Natural and recombinant rat CXCL4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,V30-S105
Gene name: Platelet factor 4 / platelet factor 4
Protein name: Platelet factor 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|205|1658|5065|199|1676|4964
Standard deviation|8.81|72.95|379.87|9.35|87.15|416.97
CV(%)|4.3%|4.4%|7.5%|4.7%|5.2%|8.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 205 | 211 | 226 | 204 | 211 | 8.78 | 4.1% |
| Sample 2 | 1658 | 1447 | 1691 | 1522 | 1579 | 99.31 | 6.2% |
| Sample 3 | 5065 | 4548 | 5213 | 4497 | 4830 | 313.17 | 6.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1651-CAP|96-well plate precoated with anti- rat CXCL4 antibody|1
EK1651-ST|Lyophilized recombinant rat CXCL4 standard|10ng/tube×2
EK1651-DA|Biotinylated anti- rat CXCL4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
