Description
Boster’s rat CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD86 has been precoated onto 96-well plates. Standards(NSO, V29-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD86 amount of sample captured in plate.
Synonyms: Activation B7 2 antigen|B7 2|B70|B72|BU63|CD28LG2|CD86 antigen (CD28 antigen ligand 2 B7 2 antigen)|CD86 molecule|CTLA 4 counter receptor B7.2|FUN 1|FUN1|T lymphocyte activation antigen CD86 precursor
Species: Rat CD86
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat CD86
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, V29-I250
Gene name: T-lymphocyte activation antigen CD86 / CD86 molecule
Protein name: T-lymphocyte activation antigen CD86
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|179|612|1878|193|658|1765
Standard deviation|10.2|44.67|110.8|13.89|51.98|112.96
CV(%)|5.7%|7.3%|5.9%|7.2%|7.9%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 179 | 159 | 158 | 184 | 170 | 11.64 | 6.8% |
| Sample 2 | 612 | 691 | 664 | 617 | 646 | 32.96 | 5.1% |
| Sample 3 | 1878 | 1659 | 1901 | 1936 | 1843 | 108.5 | 5.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0712-CAP|96-well plate precoated with anti- rat CD86 antibody|1
EK0712-ST|Lyophilized recombinant rat CD86 standard|10ng/tube×2
EK0712-DA|Biotinylated anti- rat CD86 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
