Description
Boster’s rat Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(E.coli, I19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Adiponectin amount of sample captured in plate.
Synonyms: 30 kDa adipocyte complement related protein|_x000D_30 kDa adipocyte complement-related protein|_x000D_ACDC|_x000D__x000D__x000D_ACRP30|_x000D_ADIPO_HUMAN|_x000D_Adipocyte|_x000D_Adipocyte C1q and collagen domain containing protein|_x000D_Adipocyte complement related 30 kDa protein|_x000D_Adipocyte complement related protein of 30 kDa|_x000D_Adipocyte complement-related 30 kDa protein|_x000D_Adiponectin|_x000D_Adipoq|_x000D_Adipose most abundant gene transcript 1|_x000D_Adipose most abundant gene transcript 1 protein|_x000D_Adipose specific collagen like factor|_x000D_ADIPQTL1|_x000D_ADPN|_x000D_apM-1|_x000D_APM1|_x000D_C1q and collagen domain-containing protein|_x000D_GBP28|_x000D_Gelatin binding protein|_x000D_Gelatin binding protein 28|_x000D_Gelatin-binding protein
Species: rat Adiponectin
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat Adiponectin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, I19-N244
Gene name: Adiponectin / adiponectin, C1Q and collagen domain containing
Protein name: Adiponectin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|292|1705|4269|265|1626|4075
Standard deviation|16.93|126.17|170.76|19.61|133.33|167.07
CV(%)|5.8%|7.4%|4%|7.4%|8.2%|4.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 292 | 307 | 293 | 277 | 292 | 10.61 | 3.6% |
| Sample 2 | 1705 | 1526 | 1807 | 1787 | 1706 | 110.86 | 6.4% |
| Sample 3 | 4269 | 4351 | 4667 | 4673 | 4490 | 182.33 | 4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1327-CAP|96-well plate precoated with anti- rat Adiponectin antibody|1
EK1327-ST|Lyophilized recombinant rat Adiponectin standard|10ng/tube×2
EK1327-DA|Biotinylated anti- rat Adiponectin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
