An aminonucleoside antibiotic that blocks protein synthesis in both prokaryotes and eukaryotes by causing premature termination of nascent polypeptide chains; used in assays for peptide-bond formation and elongation and also often used as selective agent in cell culture systems

Puromycin (hydrochloride) – 500 mg

Brand:
Cayman
CAS:
58-58-2
Storage:
-20
UN-No:
Non-Hazardous - 1230 / 3

Puromycin is an aminonucleoside antibiotic, derived from S. alboniger, that blocks protein synthesis in both prokaryotes and eukaryotes by causing premature termination of nascent polypeptide chains.{21743} A portion of the puromycin molecule resembles the 3′ end of aminoacylated tRNA.{21742} It enters the A site of the ribosome and attaches to the growing polypeptide chain at the peptidyl-transferase center, causing the formation of a puromycylated nascent chain and premature chain release.{21744} The 3′ position contains an amide linkage instead of the normal ester linkage of tRNA, making it much more resistant to hydrolysis and halting the activity of the ribosome.{21744} These properties of puromycin have been exploited in assays for peptide-bond formation and elongation and also the antibiotic is often used as selective agent in cell culture systems.  

 

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Description

An aminonucleoside antibiotic that blocks protein synthesis in both prokaryotes and eukaryotes by causing premature termination of nascent polypeptide chains; used in assays for peptide-bond formation and elongation and also often used as selective agent in cell culture systems

Formal name: 3′-[[2S-amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3′-deoxy-N,N-dimethyl-adenosine, dihydrochloride

Synonyms:  CL 13,900|CL 16,536|NSC 3055|PDH

Molecular weight: 544.4

CAS: 58-58-2

Purity: ≥98%

Formulation: A crystalline solid

Product Type|Biochemicals|Antibiotics||Product Type|Biochemicals|Natural Products|Microbial Metabolites||Research Area|Epigenetics, Transcription, & Translation|RNA, DNA, & Protein Synthesis

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