Reactivates AChE that has been deactivated by binding of organophosphates to its esteratic site; binds to the anionic site of AChE and displaces the phosphate from the esteratic site through formation of phosphate-pralidoxime conjugates; reactivates paraoxon- and diisopropyl fluorophosphate-inactivated human RBC AChE with IC50 shifts of 0.3 and 0.8 nM per μM of pralidoxime

Pralidoxime (chloride) – 10 g

Brand:
Cayman
CAS:
51-15-0
Storage:
-20
UN-No:
Non-Hazardous - /

Pralidoxime reactivates acetylcholinesterase (AChE) that has been deactivated by binding of organophosphates to its esteratic site.{37288} Pralidoxime binds to the anionic site of AChE and displaces the phosphate from the esteratic site through formation of phosphate-pralidoxime conjugates. It reactivates paraoxon- and diisopropyl fluorophosphate-inactivated human red blood cell (RBC) AChE with IC50 shifts of 0.3 and 0.8 nM per μM of pralidoxime, respectively.{37289},{37290} At a concentration of 10 μM, it reactivates human RBC AChE that has been inactivated by chlorpyrifos (Item No. 21412), diazinon (Item No. 23769), and malathion (Item No. 22998) by 17, 61, and 36%, respectively.{37291} Pralidoxime binds to sarin-bound hAChE (Kd = 25.72 μM) and inhibits sarin-induced AChE deactivation (IC50 = 1.21 mM) in hemoglobin-free erythrocyte ghosts.{37292} Formulations containing pralidoxime have been used to treat organophosphate poisoning.{37288}  

 

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SKU: 23666 - 10 g Category:

Description

Reactivates AChE that has been deactivated by binding of organophosphates to its esteratic site; binds to the anionic site of AChE and displaces the phosphate from the esteratic site through formation of phosphate-pralidoxime conjugates; reactivates paraoxon- and diisopropyl fluorophosphate-inactivated human RBC AChE with IC50 shifts of 0.3 and 0.8 nM per μM of pralidoxime, respectively; at a concentration of 10 μM, reactivates human RBC AChE that has been inactivated by chlorpyrifos, diazinon, and malathion by 17, 61, and 36%, respectively; binds to sarin-bound hAChE (Kd = 25.72 μM) and inhibits sarin-induced AChE deactivation (IC50 = 1.21 mM) in hemoglobin-free erythrocyte ghosts

Formal name: 2-[(hydroxyimino)methyl]-1-methyl-pyridinium, monochloride

Synonyms:  2-PAM

Molecular weight: 172.6

CAS: 51-15-0

Purity: ≥95%

Formulation: A crystalline solid

Product Type|Biochemicals|Small Molecule Activators||Research Area|Neuroscience||Research Area|Toxicology|Environmental

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