PPARs are ligand-activated transcription factors belonging to the large superfamily of nuclear receptors. PPARα primarily activates genes encoding proteins involved in fatty acid oxidation

PPARα Transcription Factor Assay Kit – 96 wells

Brand:
Cayman
Storage:
-80

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.{10561} The Cayman Chemical PPARα Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARα binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARα is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.  

 

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SKU: 10006915 - 96 wells Category:

Description

PPARs are ligand-activated transcription factors belonging to the large superfamily of nuclear receptors. PPARα primarily activates genes encoding proteins involved in fatty acid oxidation, while PPARγ primarily activates genes directly involved in lipogenic pathway and insulin signalling. Members of the PPAR family are important direct targets of many antidiabetic and hypolipidemic drugs. Cayman’s PPAR Transcription Factor Assays are a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

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Synonyms:  Peroxisome Proliferator-activated Receptor α Transcription Factor Assay Kit

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Origin: Animal/Rabbit|Animal/Bovine|Animal/Goat

Product Type|Assay Kits|Colorimetric Assays||Product Type|Assay Kits|ELISA||Research Area|Endocrinology & Metabolism|Hormones & Receptors|PPARs

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