A labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction; can be used in common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE; has been used successfully in conjunction with Daz-1 or Daz-2 to label and detect sulfenic acid sites in proteins

Phosphine-biotin – 1 mg

Brand:
Cayman
CAS:
608514-42-7
Storage:
-20
UN-No:
Non-Hazardous - /

Labeling reactive centers of various types in cells with specific site-directed probes is a common method to explore both function and biochemical modification of proteins. The popular click chemistry method of protein labeling employs use of a reaction between an azido group and an alkyne on complimentary pairs of a specific reactive probe and a labeling agent (i.e. a tag) such as biotin or a fluorophore. The Staudinger ligation is an alternative to the click chemistry reaction in which a phosphine-labeled molecule reacts with an azido group on the opposing molecule of interest. Phosphine biotin is a labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction. Modified proteins can be detected using common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE. For example, phosphine-biotin has been used successfully in conjunction with DAz-1 or DAz-2 to label and detect sulfenic acid sites in proteins.{16660,16658}  

 

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Description

A labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction; can be used in common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE; has been used successfully in conjunction with Daz-1 or Daz-2 to label and detect sulfenic acid sites in proteins

Formal name: 2-(diphenylphosphino)-4-[21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1,17-dioxo-6,9,12-trioxa-2,16-diazaheneicos-1-yl]-benzoic acid, methyl ester

Synonyms:  Click Tag™ Phosphine-biotin

Molecular weight: 792.9

CAS: 608514-42-7

Purity: ≥95%

Formulation: A crystalline solid

Application|Click Chemistry||Product Type|Biochemicals|Labeling & Detection|Reactive Probes||Research Area

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