A selective

Paprotrain – 5 mg

Brand:
Cayman
CAS:
57046-73-8
Storage:
-20
UN-No:
Non-Hazardous - /

Mitotic kinesin-like protein 2 (MKLP-2) is an N-terminal motor from the kinesin-6 family that is essential for normal cleavage furrow ingression and cytokinesis. Its activity mediates the relocation of the chromosome passenger proteins Aurora B kinase and survivin from the centromeres to the central spindle in anaphase cells in order to facilitate cytokinesis, avoiding the generation of binucleated cells.{22151} Paprotrain, PAssenger PROteins TRAnsport INhibitor, is the first known inhibitor of MKLP-2. It is a cell-permeable, reversible inhibitor uncompetitive with ATP (Ki = 3.4 μM) and noncompetitive with microtubules (Ki = 1.6 μM), demonstrating ATPase inhibition of basal and microtubule-stimulated activities with IC50 values of 1.35 and 0.83 μM, respectively.{22150} Paprotrain does not inhibit twelve additional kinesins, including the closely related kinesin-6 motors MKLP-1 and MPP1.{22150} Incubation with 10-50 μM paprotrain results in binucleated cells, perturbing relocation of Aurora B kinase and survivin without affecting microtubule polymerization.{22150}  

 

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SKU: 10524 - 5 mg Category:

Description

A selective, cell-permeable, reversible inhibitor of MKLP-2 (IC50s = 0.83-1.35 μM); treatment with 10-50 μM results in binucleated cells, perturbing relocation of Aurora B kinase and survivin to the central spindle in anaphase cells without affecting microtubule polymerization

Formal name: αZ-(3-pyridinylmethylene)-1H-indole-3-acetonitrile

Synonyms:  Passenger Proteins Transport Inhibitor

Molecular weight: 245.3

CAS: 57046-73-8

Purity: ≥95%

Formulation: A crystalline solid

Product Type|Biochemicals|Small Molecule Inhibitors||Research Area|Cell Biology|Cytoskeleton & Motor Proteins

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