p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies.{34226} Substrates phosphorylated with adenosine 5′-(γ-thio)-triphosphate (ATPγS; Item No. 14957) or N6-benzyl-ATPγS can be alkylated by PNBM.{34227,34228} The tagged substrates can be isolated by immunoprecipitation or immunoaffinity purification, or they can be detected by immunoblotting.
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A reagent that is used to alkylate thiophosphates
Formal name: 4-nitro-benzenemethanol, 1-methanesulfonate
Synonyms: para-Nitrobenzyl mesylate|4-Nitrobenzyl mesylate|PNBM
Molecular weight: 231.2
CAS: 39628-94-9
Purity: ≥99%
Formulation: A solid
Application|Immunoprecipitation||Application|Western Blot||Product Type|Biochemicals||Research Area
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