NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately

Nitrate/Nitrite Colorimetric Assay Kit (LDH method) – 96 wells

Brand:
Cayman
Storage:
-20

NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. There are two ways to get around this limitation. Vanishingly small amounts of NADPH can be used, in conjunction with a catalytic system for recycling the spent NADP+ back to NADPH. This is the system used in Cayman’s first Nitrate/Nitrite Colorimetric Assay Kit (Item No. 780001). It works well for the analysis of fluids such as plasma and urine, and is also available in a high sensitivity fluorometric version (Item No. 780051) for the detection of low levels of nitrate and nitrite. However, this method cannot be used to analyze nitrate and nitrite from an in vitro NOS assay in which excess NADPH has been added. The second way to handle the NADPH interference problem is to go ahead and use an excess of NADPH. An additional step is added to the protocol to remove the excess NADPH. This is the avenue we have taken in the development of this nitrate/nitrite assay kit. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH. The kit is particularly well suited to measurements of NOS activity in vitro. High throughput screening applications using recombinant nitric oxide synthase (NOS) preparations can be quickly and accurately assayed with the LDH method kit. Each kit contains assay buffer, nitrate reductase, cofactors preparation, nitrate standard, lactate dehydrogenase, Griess Reagent R1, Griess Reagent R2, a 96 well plate, and complete instructions.  

 

Available on backorder

Add to quotes
SKU: 760871 - 96 wells Category:

Description

NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a NOS-catalyzed reaction, thereby making the assay particularly well suited to measurements of NOS activity in vitro. High throughput screening applications using recombinant NOS preparations can be quickly and accurately assayed with the LDH method kit.

Formulation:

Formal name:

Synonyms:  Nitric Oxide Metabolite Detection Kit

Host:

Imunogen:

Applications: 

Clone:

Purity:

Origin: Animal/Rabbit

Product Type|Assay Kits|Colorimetric Assays||Product Type|Assay Kits|Detection Kits||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen|eNOS||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen|iNOS||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen|nNOS

Related products