Description
NF-κB p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-κB complex and is encoded by the RELA gene in humans.{53059} It is composed of an N-terminal Rel homology domain, which contains the nuclear localization signal (NLS), and mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal transactivation domains that are subject to a variety of post-translational modifications and regulate the transcriptional activity of p65.{53059,53060} NF-κB p65 regulates the expression of a large number of genes in response to inflammatory and environmental cues that play critical roles in innate and adaptive immunity and cellular differentiation.{53060} Silencing of Rela induces tumor cell apoptosis in a murine Lewis lung carcinoma model, and RELA silencing in THP-1 monocytes decreases secreted levels of IL-1β and TNF-α induced by LPS.{53061,53062} Genome-wide deletion of Rela in mice is embryonic lethal.{5321} NF-κB p65 is overexpressed in the inflamed joints of patients with rheumatoid arthritis, and naïve CD4 T cells isolated from the whole blood of patients with multiple sclerosis have increased phosphorylation of NF-κB p65.{53065,53066} Cayman’s NF-ĸB (p65) Polyclonal Antibody (aa 2-17) can be used for Western blot (WB) applications. The antibody recognizes amino acids 2-17 of NF-κB (p65) at 60 kDa from human, chimpanzee, and monkey samples.
Synonyms: Nuclear Factor NF-κB (p65) subunit|Transcription Factor p65
Immunogen: Synthetic peptide from the N-terminal region of human NF-κB (p65)
Formulation: 100 µg of protein G-purified IgG
Isotype:
Applications: WB
Origin: Animal/Rabbit
Stability: 365 days
Application|Western Blot||Product Type|Antibodies|Polyclonal Antibodies||Research Area|Cancer|Cell Death|Apoptosis||Research Area|Cancer|Cell Signaling|NF-κB Signaling||Research Area|Cancer|Transcription Factors|NF-κB||Research Area|Cell Biology|Cell Signaling|NF-κB Signaling||Research Area|Epigenetics, Transcription, & Translation|Transcription Factors||Research Area|Immunology & Inflammation|Adaptive Immunity||Research Area|Immunology & Inflammation|Autoimmunity|Rheumatoid Arthritis||Research Area|Immunology & Inflammation|Innate Immunity|Pattern Recognition||Research Area|Immunology & Inflammation|Innate Immunity|STING||Research Area|Infectious Disease
