Description
Boster’s mouse TNFSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF18 has been precoated onto 96-well plates. Standards(NSO, T47-S173) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF18 amount of sample captured in plate.
Synonyms: Activation inducible TNF related ligand|Activation-inducible TNF-related ligand|AITRL|GITRL|Glucocorticoid induced TNF related ligand|Glucocorticoid induced TNFR related protein ligand|Glucocorticoid-induced TNF-related ligand|hGITRL|MGC138237|TL6|TNF18_HUMAN|TNFSF18|Tumor necrosis factor (ligand) superfamily member 18|Tumor necrosis factor ligand superfamily member 18
Species: mouse TNFSF18
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse TNFSF18
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, T47-S173
Gene name: Tumor necrosis factor ligand superfamily member 18 / tumor necrosis factor (ligand) superfamily, member 18
Protein name: Tumor necrosis factor ligand superfamily member 18
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|56|252|852|60|250|921
Standard deviation|3.24|17.38|43.45|3.96|18.5|62.62
CV(%)|5.8%|6.9%|5.1%|6.6%|7.4%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 54 | 62 | 59 | 57 | 3.03 | 5.3% |
| Sample 2 | 252 | 248 | 247 | 221 | 242 | 12.26 | 5% |
| Sample 3 | 852 | 865 | 890 | 949 | 889 | 37.23 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1308-CAP|96-well plate precoated with anti- mouse TNFSF18 antibody|1
EK1308-ST|Lyophilized recombinant mouse TNFSF18 standard|10ng/tube×2
EK1308-DA|Biotinylated anti- mouse TNFSF18 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
