Description
Boster’s mouse TNFRSF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF4 has been precoated onto 96-well plates. Standards(NSO, V20-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF4 amount of sample captured in plate.
Synonyms: ACT35|ACT35 antigen|CD134|CD134 antigen|Lymphoid activation antigene ACT35|OX40|OX40 antigen|OX40 cell surface antigen|OX40 homologue|OX40L receptor|TAX transcriptionally-activated glycoprotein 1 receptor|TNFRSF4|TNR4_HUMAN|Tumor necrosis factor receptor superfamily member 4|TXGP1L
Species: Mouse TNFRSF4
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse TNFRSF4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, V20-P211
Gene name: Tumor necrosis factor receptor superfamily member 4 / tumor necrosis factor receptor superfamily, member 4
Protein name: Tumor necrosis factor receptor superfamily member 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|100|233|894|97|255|891
Standard deviation|5.8|14.91|39.33|5.72|16.32|43.65
CV(%)|5.8%|6.4%|4.4%|5.9%|6.4%|4.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 100 | 92 | 86 | 86 | 91 | 5.74 | 6.3% |
| Sample 2 | 233 | 215 | 231 | 255 | 233 | 14.23 | 6.1% |
| Sample 3 | 894 | 988 | 987 | 983 | 963 | 39.88 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0998-CAP|96-well plate precoated with anti- mouse TNFRSF4 antibody|1
EK0998-ST|Lyophilized recombinant mouse TNFRSF4 standard|10ng/tube×2
EK0998-DA|Biotinylated anti- mouse TNFRSF4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
