Description
Boster’s mouse TNFRSF17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF17 has been precoated onto 96-well plates. Standards(NSO, M1-T49) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF17 amount of sample captured in plate.
Synonyms: B cell maturation antigen|B cell maturation factor|B cell maturation protein|B-cell maturation protein|BCM|BCMA|CD 269|CD269|CD269 antigen|TNFRSF 17|TNFRSF17|TNR17_HUMAN|Tumor necrosis factor receptor member 17|Tumor necrosis factor receptor superfamily member 17
Species: Mouse TNFRSF17
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse TNFRSF17
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, M1-T49
Gene name: Tumor necrosis factor receptor superfamily member 17 / tumor necrosis factor receptor superfamily, member 17
Protein name: Tumor necrosis factor receptor superfamily member 17
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|82|288|757|87|276|787
Standard deviation|3.69|11.52|56.77|4.87|12.97|72.4
CV(%)|4.5%|4%|7.5%|5.6%|4.7%|9.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 82 | 85 | 83 | 96 | 86 | 5.59 | 6.5% |
| Sample 2 | 288 | 282 | 296 | 300 | 291 | 6.98 | 2.3% |
| Sample 3 | 757 | 861 | 852 | 799 | 817 | 42.08 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0662-CAP|96-well plate precoated with anti- mouse TNFRSF17 antibody|1
EK0662-ST|Lyophilized recombinant mouse TNFRSF17 standard|10ng/tubex2
EK0662-DA|Biotinylated anti- mouse TNFRSF17 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
