Description
Boster’s mouse TNFRSF12A/TWEAKR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF12A/TWEAKR has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: E.coli, E28-P801) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF12A/TWEAKR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Fibroblast growth factor-regulated protein 2; Tweak-receptor; TweakR; CD266; Tnfrsf12a; Fgfrp2; Fn14
Species: Mouse TNFRSF12A/TWEAKR
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant mouse TNFRSF12A/TWEAKR
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, E28-P80
Gene name: Tumor necrosis factor receptor superfamily member 12A / tumor necrosis factor receptor superfamily, member 12a
Protein name: Tumor necrosis factor receptor superfamily member 12A
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|300|2167|4789|307|2379|4841
Standard deviation|18.3|143.02|258.6|13.2|114.19|363.08
CV(%)|6.1%|6.6%|5.4%|4.3%|4.8%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 300 | 325 | 308 | 330 | 315 | 12.21 | 3.8% |
| Sample 2 | 2167 | 2176 | 2072 | 2103 | 2129 | 43.52 | 2.0% |
| Sample 3 | 4789 | 4265 | 4165 | 4933 | 4538 | 328.89 | 7.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1754-CAP|96-well plate precoated with anti- mouse TNFRSF12A/TWEAKR antibody|1
EK1754-ST|Lyophilized recombinant mouse TNFRSF12A/TWEAKR standard|10ng/tube×2
EK1754-DA|Biotinylated anti- mouse TNFRSF12A/TWEAKR antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
