Description
Boster’s mouse TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF beta1 amount of sample captured in plate.
Synonyms: Camurati-Engelmann disease|_x000D_CED|_x000D_DPD1|_x000D__x000D__x000D_LAP|_x000D_Latency-associated peptide|_x000D_TGF beta 1 protein|_x000D_TGF-beta-1|_x000D_TGFB|_x000D_tgfb1|_x000D_TGFB1_HUMAN|_x000D_TGFbeta|_x000D_Transforming growth factor beta 1
Species: Mouse TGF beta 1
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant mouse TGF beta1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: CHO, A279-S390
Gene name: Transforming growth factor beta-1 / transforming growth factor, beta 1
Protein name: Transforming growth factor beta-1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|36|169|499|33|183|484
Standard deviation|1.47|11.66|31.43|1.55|12.99|34.36
CV(%)|4.1%|6.9%|6.3%|4.7%|7.1%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 39 | 42 | 38 | 38 | 2.16 | 5.6% |
| Sample 2 | 169 | 169 | 184 | 172 | 173 | 6.18 | 3.5% |
| Sample 3 | 499 | 442 | 521 | 490 | 488 | 28.85 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0515-CAP|96-well plate precoated with anti- mouse TGF beta 1 antibody|1
EK0515-ST|Lyophilized recombinant mouse TGF beta 1 standard|10ng/tubex2
EK0515-DA|Biotinylated anti- mouse TGF beta 1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
