Description
Boster’s mouse SDF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SDF-1 has been precoated onto 96-well plates. Standards(E.coli, K22-M93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SDF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SDF-1 amount of sample captured in plate.
Synonyms: C-X-C motif chemokine 12|_x000D_Chemokine (C X C motif) ligand 12|_x000D_CXCL12|_x000D__x000D__x000D_hIRH|_x000D_Intercrine reduced in hepatomas|_x000D_IRH|_x000D_PBSF|_x000D_Pre B cell growth stimulating factor|_x000D_SCYB12|_x000D_SDF 1|_x000D_SDF 1 beta|_x000D_SDF 1b|_x000D_SDF1|_x000D_SDF1 alpha (3-67)|_x000D_SDF1 beta (3-72)|_x000D_SDF1A|_x000D_SDF1B|_x000D_Stromal cell derived factor 1|_x000D_TLSF a|_x000D_TLSF b|_x000D_TLSFa|_x000D_TLSFb|_x000D_TPAR1
Species: Mouse SDF-1
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse SDF-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: E.coli, K22-M93
Gene name: Stromal cell-derived factor 1 / chemokine (C-X-C motif) ligand 12
Protein name: Stromal cell-derived factor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|110|638|1235|117|577|1250
Standard deviation|5.72|49.12|60.51|7.48|53.66|66.25
CV(%)|5.2%|7.7%|4.9%|6.4%|9.3%|5.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 110 | 115 | 107 | 99 | 107 | 5.8 | 5.4% |
| Sample 2 | 638 | 625 | 738 | 699 | 675 | 45.86 | 6.7% |
| Sample 3 | 1235 | 1269 | 1293 | 1382 | 1294 | 54.42 | 4.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0500-CAP|96-well plate precoated with anti- mouse SDF-1 antibody|1
EK0500-ST|Lyophilized recombinant mouse SDF-1 standard|10ng/tubex2
EK0500-DA|Biotinylated anti- mouse SDF-1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
